Table 4 Strains used in this study
Strain | Genotype | Reference |
|---|---|---|
E. coli DH5α | F- φ80lac ZΔM15, Δ(lacZYA-argF) U169, deoR, recA1, endA1, hsdR17(rk−mk+) supE44, λ-, thi-1, gyrA96, relA1 phoA | Novagen |
BY4742 | MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0 | ATCC |
BY-I | PADH1-ERG20-TTPI1, PHXT7-ERG9-TPGIT, PPGI-ERG1-TADH, PRPL8A-PgDS-TCYC1, PADH1-CYP716A47-46ATR1-TFBA1, PTDH3-tHMG1-TPDC1 cassettes and HIS5 marker gene were integrated into delta17 site of BY4742 | This study |
BY-II | PENO2-ERG12-TCPS1, PTEF2-ERG13-TIDP1, PGPM1-MVD1-THIS5, PTPI1-ERG8-TPRM5, PGDP1-IDI1-TPRM9, PTDH3-tHMG1-TTDH2, PTEF1-ERG10-TSPG5 cassettes and LEU2 marker gene were integrated into delta15 site of BY-I | This study |
BY-III-1 | BY-II, PINO2::PHXT7 | This study |
BY-III-2 | BY-II, PINO2::PPGK1 | This study |
BY-III-3 | BY-II, PINO2::PTDH3 | This study |
BY-III-4 | BY-II, PINO2::PTEF1 | This study |
BY-III-5 | BY-II, PERG7::PHXT1 | This study |
BY-IV | BY-III-4, PERG7::PHXT1 | This study |
BY-V | BY-IV, LPP1::PPGK1-UPC2.1 | This study |