High-yield production of protopanaxadiol from sugarcane molasses by metabolically engineered Saccharomyces cerevisiae

Table 2 PPD production of strain BY-V in fed-batch culture

Carbon source	Feeding strategy	PPD^a (mg/g DCW)	PPD (mg/L)	[PPD/(sugar + ethanol)] (mg/g)	Time (h)
Glucose	NA^b	78.13 ± 0.38^c	563.60 ± 1.65	18.79 ± 0.23	72
Molasses	NA	53.04 ± 0.30	402.22 ± 7.39	13.11 ± 0.16	72
Glucose	G + N^d	41.29 ± 0.78	841.09 ± 2.16	8.14 ± 0.56	96
Molasses	M + N	64.33 ± 0.54	556.26 ± 4.90	11.13 ± 0.44	120
Glucose	G + E + N	77.01 ± 0.17	1320.69 ± 42.51	14.67 ± 0.68	120
Molasses	M + E + N	79.92 ± 0.19	1251.56 ± 12.54	15.63 ± 0.83	168
Glucose	E + N	95.15 ± 0.96	1323.22 ± 13.13	19.41 ± 0.75	120
Molasses	E + N	106.55 ± 0.91	1553.68 ± 18.72	22.79 ± 1.06	168

^aPPD yields represented the total amounts of intracellular and extracellular extraction
^bNA: Not applicable
^cAll data are presented as mean ± standard deviation of biological triplicates
^dG, N, M and E represented glucose, nitrogen, molasses and ethanol, respectively

ISSN: 1475-2859