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Table 4 Ingredients in the DMB [11] and Wolfe’s [12, 13] trace-element solutions

From: Medium development and production of carotenoids and exopolysaccharides by the extremophile Rhodothermus marinus DSM16675 in glucose-based defined media

Ingredients (DMB trace)

Amount (per liter DMB-trace)

Ingredients (modified Wolfe’s trace)

Amount (per liter modified Wolfe’s trace)

Nitrilotriacetic acid

12.8 g

Nitrilotriacetic acid

1.5 g

  

MgSO4.7H2O

3.0 g

  

NaCl

1.0 g

FeCl2 .4H2O

1 g

FeSO4·7H2O

0.1 g

MnCl2 .4H2O

0.5 g

MnSO4·H2O

0.5 g

CoCl2 .6H2O

0.3 g

CoCl2·6H2O

0.1 g

  

CaCl2

0.1 g

CuCl4 .2H2O

0.05 g

ZnSO4·7H2O

CuSO4·5H2O

AlK(SO4)2·12H2O

0.1 g

0.01 g

0.01 g

Na2MoO4 .2H2O

0.05 g

Na2MoO4·2H2O

0.01 g

H3BO3

0.02 g

H3BO3

0.01 g

NiCl2 .6H2O

0.02 g

  

Distilled water

Up to 1 L

Distilled water

Up to 1 L

Adjusting the pH to 7 by NaOH (6 M)

Adjusting the pH to 7 by KOH (2 M)