Table 1 Macroelements and additives in the DMB [11] and the modified Wolfe’s media [12, 13]

Nitrilotriacetic acid

2.01 g

Tris

4.85 g

CaSO₄.2H₂O

0.4 g

CaSO₄.2H₂O

0.04 g

MgCl₂.6H₂O

2 g

MgCl₂.6H₂O

0.20 g

NaCl

10.0 g

NaCl

10.0 g

KH₂PO₄

0.27 g

KH₂PO₄

0.30 g

Na₂HPO₄

0.28 g

K₂HPO₄

0.50 g

NH₄Cl

0.5 g

NH₄Cl

0.5 g

Ferric citrate (C₆H₅FeO₇)

0.06 g

Trace elements solution DMB (Table 4)

5 mL

Wolfe’s trace element solution (Table 4)

5 mL

Vitamins solution DMB (Table 5)

1 mL

Wolfe’s vitamins solution (Table 5)

5 mL

Asparagine

0.002 g

Yeast extract

0.05 g

Glutamine

0.002 g

Distilled water

Up to 1 L

Distilled water

Up to 1 L

Adjust the pH to 7.2 by NaOH (6 M)

Adjust the pH to 7.2 by NaOH (6 M)