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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Formation of intracellular vesicles within the Gram+ Lactococcus lactis induced by the overexpression of Caveolin-1β

Fig. 1

Expression of HTC in L. lactis after 4 h post-induction by nisin. Total membrane proteins (5 µg for A, 5 and 10 µg for B) were separated in a 12% SDS–PAGE and analyzed by Western blot performed using either an antibody specific to caveolin-1 (A; 1/7500) or an HRP-conjugate specific to the His-tag affinity tag (B; 1/5000). A positive control protein containing caveolin-1β (C + ; 15 µg) and the band of 75 kDa of the molecular weight from Bio-Rad (constitutively His-tagged) were used to estimate the expression levels of the recombinant proteins. H means membrane proteins derived from bacteria containing the recombinant pNZ-HTC vector, C- means crude membrane proteins derived from control bacteria containing the empty pNZ8148 vector. o,d,m correspond respectively to oligomer, dimer and monomer. Western blot images are merged images of both colorimetric analysis of membranes revealing the molecular weights and chemiluminescent analysis revealing only some molecular weight bands

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