Fig. 5
From: Scale-up of an amoeba-based process for the production of the cannabinoid precursor olivetolic acid
Scale-up of the OA production process from shake flask to 300-L STR based on εmax. After expanding the pre-culture regime from 1.6-L over 7-L to 75-L STR, cells were transferred into a 300-L STR at a cell density of 1 × 107 cells/mL. A Online parameters of D. discoideum pChR7 batch fermentation including carbon dioxide transfer rate (CTR-STR, red solid; CTR-shake flask as reported in Reimer et al., 2022, red dotted), dissolved oxygen tension (DOT, black solid) and sulfuric acid consumption (black dotted) were monitored. B Offline parameters such as cell dry weight (CDW, grey), cell number (blue), glucose consumption (black), viability (violet) and olivetolic acid production (OA-STR, green solid; OA-shake flask, green dotted) are depicted over the course of the fermentation. Culture conditions: 300-L STR; HL5 medium with 10 g/L glucose, 20 µg/mL G418 and 20 µg/mL doxycycline; pH = 6.5 ± 0.05 was maintained by the addition of 10% H2SO4; tip speed: 2.62 m/s; stirring rate = 255 rpm; aeration rate: 0.3 vvm; temperature 22 °C