Fig. 2
From: Scale-up of an amoeba-based process for the production of the cannabinoid precursor olivetolic acid
Influence of εmax on growth of the D. discoideum pChR7 strain. During the lag phase, the stirrer tip speed was kept constant at 0.35 m/s for 52 h. After reaching the exponential growth phase (black arrow), the stirrer speed was changed as indicated. The growth behavior was monitored by measuring the carbon dioxide transfer rate (CTR) (A), dissolved oxygen tension (DOT) (B), the cell dry weight (CDW) (C) and glucose consumption (D) for the different stirrer tip speeds. Culture conditions: 1.6-L STR; HL5 medium with 10 g/L glucose, 20 µg/mL G418 and 20 µg/mL doxycycline; aeration rate: 0.3 vvm; temperature 22 °C; pH = 6.5 ± 0.05