Table 2 Plasmids and strains used in this study
Plasmids or strains | Genotype | Source |
|---|---|---|
Plasmids | ||
 pETDuet-1 | Double T7 promoters, pBR322 ori, AmpR | Novagen |
 pET32a (+) | T7 promoter, F1 ori, AmpR | Novagen |
 pET32a-ScCGT1 | pET32a-ScCGT1 | This study |
 pET32a-ScCGT1-P164T | pET32a-ScCGT1-P164T | This study |
 pETDuet-1-CjFNS I/F2H | pETDuet-1 carrying CjFNS I/F2H from Conocephalum japonicum | This study |
 pETDuet-1-CjFNS I/F2H-ScCGT1 | pETDuet-1 carrying CjFNS I/F2H from C. japonicum and ScCGT1 | This study |
 pETDuet-1-CjFNS I/F2H-ScCGT1-P164T | pETDuet-1 carrying CjFNS I/F2H from C. japonicum and ScCGT1-P164T | This study |
 pACYCDuet-cscB-Basp-UgpA | pACYCDuet carrying cscB from E. coli W, Basp from Bifidobacterium adolescentis, and ugpA from B. bifidum | [32] |
 pACYCDuet-Pgm-GalU | pACYCDuet carrying pgm and galU from E. coli K12 | [32] |
Strains | ||
 E. coli DH5α |  | Takara |
 E. coli BL21 |  | Takara |
 W1 | BL21(DE3) carrying pET32a-ScCGT1 | This study |
 W2 | BL21(DE3) carrying pET32a-ScCGT1-P164T | This study |
 EA1 | BL21(DE3) carrying pET32a-ScCGT1 and pACYCDuet-Pgm-GalU | This study |
 EA2 | BL21(DE3) carrying pET32a-ScCGT1-P164T and pACYCDuet-cscB-Basp-UgpA | This study |
 EA3 | BL21(DE3) carrying pETDuet-1-CjFNS I/F2H-ScCGT1 | This study |
 EA4 | BL21(DE3) carrying pETDuet-1-CjFNS I/F2H-ScCGT1-P164T | This study |
 EA5 | BL21(DE3) carrying pETDuet-1-CjFNS I/F2H-ScCGT1-P164T and pACYCDuet-cscB-Basp-UgpA | This study |