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Table 1 The kinetic parameters of recombinant ScCGT1 using phloretin (1) and 2-hydroxynaringenin (2) as substrate and UDP-glucose as the sugar donor

From: Identification of a flavonoid C-glycosyltransferase from fern species Stenoloma chusanum and the application in synthesizing flavonoid C-glycosides in Escherichia coli

Enzyme

Substrate

Km (μM)

Vmax (nmol mg−1 min−1)

kcat (min−1)

kcat/Km(M−1 s−1)

ScCGT1

Phl

23.8 ± 2.8

11.0 ± 0.3

0.8 ± 0.02

559.8

ScCGT1-P164T

Phl

29.8 ± 2.5

33.4 ± 0.7

2.4 ± 0.05

1357.5

ScCGT1

2-OHNA

17.5 ± 2.0

11.5 ± 0.3

0.8 ± 0.01

795.4

ScCGT1-P164T

2-OHNA

31.7 ± 2.9

39.9 ± 0.9

2.9 ± 0.06

1522.0

  1. Phl, phloretin; 2-OHNA, 2-hydroxynaringenin
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Microbial Cell Factories

ISSN: 1475-2859

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