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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Identification of a flavonoid C-glycosyltransferase from fern species Stenoloma chusanum and the application in synthesizing flavonoid C-glycosides in Escherichia coli

Fig. 7

Production of nothofagin (1a) by bioconversion in E. coli cell. A The effect of phloretin (1) concentrations ranging from 50 to 150 μM on the production of 1a. B The production of 1a in E. coli strains W1 and W2. C HPLC analysis of the reaction products of E. coli strains W1, W2, and negative control (NC) fed with 100 μM phloretin (1). D Typical negative ion MS and MS2 spectra of product 1a. E The two alternative metabolic pathways are used for UDP-glucose synthesis. F E. coli EA1 and EA2 produced 1a yield when fed with 100 μM phloretin (1). Data presented in the form mean ± SD (n = 3); *p < 0.05 and **p < 0.01 (MS: mass spectrometry; MS2: tandem mass spectrometry)

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