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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Identification of a flavonoid C-glycosyltransferase from fern species Stenoloma chusanum and the application in synthesizing flavonoid C-glycosides in Escherichia coli

Fig. 6

Structure-guided mutagenesis of ScCGT1. A Structure comparison analysis of ScCGT1 and GgCGT. B Comparison of the active sites of ScCGT1 and GgCGT. C The relative activities of wild-type ScCGT1 and mutants, using 1 and 2 as the substrates. The level of 1a and 2a from wild-type ScCGT1 was set as a value of 1.0. D Illustrations of docking of sugar donor (Glc) and sugar acceptor (phloretin) in the binding pocket of ScCGT1 and ScCGT1-P164T. E Location of His26 and Asp141 residues in the structure of ScCGT1. F A possible catalytic model for ScCGT1. Data presented in the form mean ± SD (n = 3); *p < 0.05 and **p < 0.01 (Glc, UDP-glucose; 1, phloretin; 2, 2-hydroxynaringenin)

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