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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Identification of a flavonoid C-glycosyltransferase from fern species Stenoloma chusanum and the application in synthesizing flavonoid C-glycosides in Escherichia coli

Fig. 4

C-glycosylation of 2-hydroxyeriodictyol (3) catalyzed by recombinant ScCGT1. A ScCGT1 catalyzed 2-hydroxyeriodictyol (3) to produce 3a, 3b, and 3c. B HPLC chromatograms of the glycosylation reactions. C Typical negative ion MS and MS2 spectra of products 3a, 3b, 3c and standards. D The putative splitting way of 3a, 3b, and 3c on MS2. S2, luteolin (dehydrated substrate); 3a, 2-hydroxyeriodictyol C-glycoside; 3b, orientin; 3c, isoorientin. (3, 2-hydroxyeriodictyol; Glc, UDP-glucose; ori, orientin; isoori, isoorientin; STD: Standard; MS: mass spectrometry; MS2: tandem mass spectrometry)

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Microbial Cell Factories

ISSN: 1475-2859

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