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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Active human full-length CDKL5 produced in the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125

Fig. 7

Effect of pathogenic CDKL5 mutations on flCDKL5 activity. a flCDKL5 variants and EB2 were co-expressed in P. haloplanktis TAC125 using TCD plasmids. After cellular lysis, flCDKL5 mutants and EB2 were detected in each crude lysate using an anti-His antibody after Western blot, as indicated by the two arrows. b Phosphorylated EB2 was detected with an anti-EB2 pSer222 antibody. c EB2 phosphorylation levels were normalized for total EB2 and flCDKL5. The results are reported as the mean of technical triplicates and the error bars represent standard deviations. a and b show Western blots representative of one experiment. * p < 0.05, ** p < 0.01, *** p < 0.001. Note: every CDKL5 construct (also the wt) harbors an M10V mutation besides the one indicated in the figure. Mutation coordinates are referred to the human CDKL5 isoform 1 (#O76039, Uniprot)

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