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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Active human full-length CDKL5 produced in the Antarctic bacterium Pseudoalteromonas haloplanktis TAC125

Fig. 4

flCDKL5 production profiles with BCD plasmids. a Schemes of flCDKL5 constructs tested with BCD plasmids. All flCDKL5 variants are N-terminally fused to a TATκ peptide for intracellular vehiculation [4]. 107 (G) has C-terminal 3xFLAG and 6xHis tags. 107 (K) has an N-terminal 6xHis and a C-terminal 3xFLAG tag. 107 (L) has tandem N-terminal 6xHis and Sumo tags, and a 3xFLAG at the C-terminus. The residue M10 that was subjected to mutation is indicated for all flCDKL5 variants. b anti-CDKL5 (left panel), anti-FLAG (middle panel), and anti-His Western blots (right panel) of P. haloplanktis TAC125 crude lysates after the expression of the 107 (G) and 107 (K) variants in monocistronic (p79C) and BCD1, BCD2 and BCD3 bicistronic configurations. c anti-CDKL5 (left panel), anti-FLAG (middle panel), and anti-His Western blots (right panel) of P. haloplanktis TAC125 crude lysates expressing multiple either wt or mutant 107 (G), 107 (K), and 107 (L) variants with BCD2 plasmids. NI not induced strain

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