Fig. 4

Effect of introducing the IUP on the synthesis of IPP and DMAPP. A Scheme of introducing the IUP into S. cerevisiae. B Comparison of intracellular IPP and DMAPP contents in S. cerevisiae control strain Sclin01, strain BY4742-MC-02 with the whole MVA pathway in both mitochondria and cytoplasm, and strain Sclin02 with the IUP. Strains Sclin01 and Sclin02 were cultured in YPS medium containing 17.5 mM isoprenol and prenol, respectively, and BY4742-MC-02 was grown in YPS medium