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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Fig. 3

Enzyme activity of CsFLS2 and CsFLS3. A HPLC chromatogram of dihydroflavonols (a and b) and flavanone (c) catalyzed by recombinant CsFLS2 and CsFLS3 in vitro. B Validation of catalytic products using Q-TOF. C Analysis of catalytic activity and preference of CsFLS substrates. (d and e) The activity of CsFLS2 and CsFLS3 proteins was determined at various temperatures (15–50 ℃) and pH (5.0–8.5). (f–i) Michaelis–Menten plots for both dihydroflavonols of recombinant CsFLS2 and CsFLS3 enzymes. K, Kaempferol; Q, Quercetin; DHQ, dihydroquercetin; DHK, dihydrokaempferol; Nar, Naringenin; Api, Apigenin

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