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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Fig. 2

Catalytic characteristics of CsFLS. A Phylogenic analysis of CsFLS. The neighbor-joining method was used to construct this tree with 1000 replicate bootstraps using MEGA6.0. The CsFLSs characterized in this work are marked with red stars. Accession numbers of proteins from other plants are shown in Additional file 5. Table S4. B Sequence alignment of CsFLSs with functionally verified FLSs in Apiaceae. The proposed residues involved in binding the dihydroquercetin substrate are marked with circles. The conserved domain of 2-ODD was underlined. Inferred amino acids for binding the ferrous iron and 2-oxoglutarate (2-OG) were marked with pentagram and lozenge, respectively. C Predicted three-dimensional structures of CsFLS2 and CsFLS3. CsFLS2 binding sites for Fe2+ and 2-OG were H218, D220, and H274, and R284 and S286, respectively. Meanwhile, H233, D235, and H289, as well as R299 and S301 are the Fe2+ and 2-OG binding sites of CsFLS3, respectively. D Prediction of dihydrokaempferol (DHK), dihydroquercetin (DHQ), and naringenin (Nar) interacted with modeling CsFLS2 (a–c) and CsFLS3 (d–f) by docking simulation

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Microbial Cell Factories

ISSN: 1475-2859

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