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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Genome-wide identification of key enzyme-encoding genes and the catalytic roles of two 2-oxoglutarate-dependent dioxygenase involved in flavonoid biosynthesis in Cannabis sativa L.

Fig. 1

Expression of gene-related flavonoid biosynthesis and flavonoid content in different tissues in C. sativa. A Schematic representation of gene expression in the flavonoid biosynthesis pathway and accumulation of flavonoids in six tissues of DiKu. Data of the transcriptional level was present by log2 (FPKM + 1). The content of flavonoids was presented by the average of peak area. B: Bract, F: Flower, L: Leaf, St: Stem, Se: Seed, R: Root. PAL: phenylalanine ammonia lyase, C4H: cinnamate 4-hydroxylase, 4CL: 4-coumaric acid: CoA ligase, CHS: chalcone synthase; CHI: chalcone isomerase, F3H: flavanone 3-hydroxylase, FNS: flavone synthase; F3′H: flavonoid 3′-hydroxylase, F3′5′H: flavonoid 3′5′-hydroxylase, FLS: flavonol synthase, OMT: O-methyltransferase, and PT: prenyltransferase. B Relative expression of selected genes in different tissues of C. sativa. n = 3. Different letter indicates significance (p < 0.05). C Accumulation of major flavonoids of C. sativa. in different tissues of different chemovars. Data are presented as the average of the peak area.TI: Terra Italia, SD: Swiss Dream, PK: Pain killer, GG: Gorilla Glue, RP: Red Pure, and DK: Dinamed Kush. D Variation in total flavonoids in different tissues of C. sativa. Results were Mean ± SE (n = 3). E Correlation analysis of the transcription abundance of genes participating in the flavonol biosynthetic pathway with contents of cannflavin A, cannflavin B, luteolin, naringenin, apigenin, kaempferol, and quercetin in C. sativa

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