Fig. 3
From: Metabolic engineering enables Bacillus licheniformis to grow on the marine polysaccharide ulvan
In-depth analysis of the capability of B. licheniformis DSM13 to degrade ulvan-derived oligosaccharides. Ulvan was digested with selected enzymes or enzyme cocktails, described before [14, 15], to produce a total of 12 different ulvan hydrolysates (Additional file 1: Fig. S4). a These vary in their mono- and oligosaccharide content based on b the enzymes used and thus provide specific carbon sources for B. licheniformis DSM13. The DSM13 strain was cultivated in M9-mineral medium supplemented with ulvan or enzyme-generated ulvan hydrolysates and OD600 was measured after 24Â h. Growth on hydrolysates UHA and UHB, which were used for further investigations, is highlighted. (PL: mix of P10_PL40; GH: mix of P33_GH105, P1_GH88; S: P36_S1_25|GH78)