Fig. 2From: Metabolic engineering enables Bacillus licheniformis to grow on the marine polysaccharide ulvanProtease production of B. licheniformis DSM13 during growth on different substrates. a Bacillus licheniformis was grown in M9-mineral salts medium supplemented with ulvan, ulvan hydrolysate (UHB) (Additional file 1: Tables S1, S3 and S4) or glucose for 80 h. Cultures without added carbon source and with or without the ulvan hydrolysing enzymes (see Additional file 1: Table S1) served as controls. Dotted lines mark time points for b determination of protease activity using the AAPF test. c Growth on UHB over 8 days with corresponding protease activity measurementsBack to article page