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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Integrated multi-omics analysis and microbial recombinant protein system reveal hydroxylation and glycosylation involving nevadensin biosynthesis in Lysionotus pauciflorus

Fig. 3

Identification of LpF6/8H by phylogenetic analysis and enzymatic tests in yeast. a Phylogenetic tree of A-type (71 clans) CYP450s in L. pauciflorus and Arabidopsis thaliana. The neighbor-joining tree was constructed using Mega 11.0 with 1000 bootstrap replicates. The respective protein names and numbers are listed in (Additional file 11: Table S6). The CYP450 members from L. pauciflorus are marked in red. b Phylogenetic tree of previously characterized flavonoid biosynthesis CYP450s and those LpCYP450s isolated in this study (red circle). c, d Identification of the enzymatic products of recombinant LpCYP82D-8 and LpCYP82D-1 proteins by UPLC and UPLC/MS/MS. The peaks marked in red were the products of in vivo yeast reaction. SCU: Scutellarein; ISOSCU: Isoscutellarein. e Relative expression of two LpCYP82D genes in different tissues determined by qRT-PCR

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Microbial Cell Factories

ISSN: 1475-2859

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