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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Integrated multi-omics analysis and microbial recombinant protein system reveal hydroxylation and glycosylation involving nevadensin biosynthesis in Lysionotus pauciflorus

Fig. 2

The KEGG analysis based on the transcriptome and putative biosynthesis pathway for nevadensin in L. pauciflorus. a Comparative analysis of the KEGG functional enrichment of differentially expressed genes (DEGs) in different groups. b The proposed pathway of nevadensin in L. pauciflorus. The heatmap highlights the patterns of expression for these genes in root, stem, leaf, and flower, with FPKM values used for normalization and red-to-blue color-coded. The relative contents of flavones in four tissues are in red-to-green color-coded. Arrow code: black solid line, evidence this conversion occurs in the plant; red dotted line, biochemically favorable step; grey dotted line, no current information as to whether or how the reaction occurs. Color code: green, confirmed enzyme; red, enzyme relevant to this study. Flavonoid backbone numbering and ring nomenclature are indicated on the apigenin structure in blue coded. Nevadensin was in a red line rectangle. F6/8H, flavone 6/8 hydroxylase; F6/8OMT, flavone 6/8-O methyltransferase; 5/7-OGT, 5/7-O Glycosyltransferase

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