Enhancement of ergothioneine production by discovering and regulating its metabolic pathway in Cordyceps militaris

Table 1 Putative ergothioneine biosynthesis genes blast from edible mushrooms by comparing with NcEgt1 and NcEgt2

Species	Egt1 - size (aa)	Methyl_EasF¹ (aa)	DinB_2² (aa)	FGE-sulfatase³ (aa)	Query Cover (%)	Per. ident⁴ (%)	Positive (%)	Egt2 – size(aa)	CsdA⁵ (aa)	Query Cover (%)	Per. ident⁴ (%)	Positive (%)
Neurospora crassa	876	36–350	391–514	573–874	100	100	100	473	77–381	100	100	100
Cordyceps militaris	890	40–337	417–544	580–887	98	56.43	70	456	72–448	94	48.78	63
Agrocybe aegerita	884	21–417	469–598	669–878	93	34.89	47	563	31–420	94	35.65	51
Agaricus bisporus	871	20–403	457–586	659–868	95	34.52	49	439	22–430	93	34.80	52
Lentinula edodes	865	20–398	452–581	654–862	99	34.37	48	447	29–420	96	33.62	54
Pleurotus ostreatus	853	5-381	438–570	640–850	96	34.64	49	445	25–425	94	34.90	52
Flammulina velutipes	819	2-362	416–541	611–816	99	35.57	47	458	34–436	96	33.40	51
Grifola frondosa	859	27–403	450–579	648–856	97	33.81	50	439	20–339	93	31.10	47
Ganoderma sinense	867	27–410	457–586	656–864	98	33.75	48	465	26–455	93	34.96	52

¹Methyl_EasF: probable methyltransferase domain, EasF family; represents about 300 amino acids with homology to S-adenosylmethionine-dependent methyltransferases
²DinB_2: DinB superfamily; The DinB family are an uncharacterized family of potential enzymes. The structure of these proteins is composed of a four-helix bundle
³FGE-sulfatase: Sulfatase-modifying factor enzyme 1; probable functions as an iron (II)-dependent oxidoreductase
⁴Per. Ident: represent percent identity of amino acid sequence between the input genes to NcEgt1 or NcEgt2
⁵CsdA: CsdA superfamily; probable selenocysteine lyase or cysteine desulfurase

ISSN: 1475-2859