Fig. 2

Dissociation of oligomers of hGH and oxidative folding. A SDS-PAGE of non-boiled and non-reduced supernatants of hGH-IBs suspensions at a concentration of 1 mg hGH/mL that were subjected to 2.4 kbar and pH 9.0 and 10.0 in the absence and in the presence of DTT (10 mM) and Arg (0.25 M); B SDS-PAGE of supernatants of hGH-IBs that were subjected to 2.4 kbar for 90 min at pH 9.0 and 10.0 in the presence of 10 mM DTT and in the presence and absence of Arg (0.25 M). For refolding, the supernatants of the centrifuged samples were diluted tenfold in Tris pH 7.4 in the presence of 2 mM GSSG, incubated and dialyzed. IB indicates the hGH-IBs, that was reduced and Red indicates a reduced soluble sample; C HPSEC analysis of the samples solubilized at pH 10.0 and treated as in B; D SDS-PAGE of samples refolded as in B in the presence of Arg and in the presence or in the absence of DTT and GSSG; E, HPSEC of samples solubilized at pH 10.0 and treated as in D