Fig. 3
Synthetic biology methods for biosynthetic regulation to increase the titer, rate, and yield (TRY) of product. A Diagram of artificial neural network-guided biosynthetic regulation. B Pathway optimization based on the promoter library containing different expression intensities. C Novel enzyme search and initial enzyme modification, optimization and protein design. C1, compound 1; C2, compound 2; C3, compound 3; C4, compound 4; E1, enzyme 1; E2, enzyme 2; E3, enzyme 3. D The process for transforming exogenous genes and exogenous pathways into engineered strains by means of plasmid construction or genomic integration. E The engineering process reduces biomass, reduces the loss of carbon sources in branching pathways, reduces metabolic competition between desired products and byproducts, and ensures the continued accumulation of the desired products