Enhanced protein translocation to mammalian cells by expression of EtgA transglycosylase in a synthetic injector E. coli strain

Table 2 Oligonucleotides used in this study

Name	Sequence (5′–3′)^a	Purpose
F_NotI_etgA	GCTTTGCGGCCGCATATTGAGTAACAACGTAAAAATGAAAAAAATAATACTGAGTATCATTCTC	Cloning of the etgA gene
R_SpeI_etgA	GTGACACTAGTTTAATCGATAATTTGCTCATTATTCTTTAA	Cloning of the etgA gene
F_SacI_RBS_EspF20	AATTCGAGCTCAAGCTTAAGAAGGAGATATACATATGCTTAATGGAATTAGTAACGCTGCTTC	Cloning of EspF20-bla
F_SacI_HindIII_bla	ATGCTGAGCTCAAGCTTAAGAAGGAGATATACATATGGGAAGCCACCCAGAAACGCTGGTGAAAGTAAAAG	Cloning of Bla
R_SpeI_Bla	CATGCACTAGTTTACCAATGCTTAATCAGTGAGGCACC	Cloning of EspF20-bla and Bla
Int_UP_sfm	TTCTTCACAAGCCTTGCGTTTGCTTGCGAC	Check etgA integration
F.check.DescN	CGGCGTACAAGAAACGCGTTATTTG	Check escN deletion
R.check.DescN	CGAAAACATAGTCTTTTTTATG	Check escN deletion
tolC-kmUP	ATGAAGAAATTGCTCCCCATTCTTATCGGCCTGAGCCTTTCTGGGTTCAGgtgtaggctggagctgcttc	Deletion of tolC
tolC-KmDWN	TCAGTTACGGAAAGGGTTATGACCGTTACTGGTGGTAGTGCGTGCGGATGcatatgaatatcctccttagt	Deletion of tolC
TolC-UP-1	CGGGAATTCTCTAGACTGCTTCACCACAAGGAATGCAAATG	Check tolC::km1
TolC-DW-1	CCGCGGATCCAAGCTTGGCCGAAGCCCCGTCGTCGTCATCA	Check tolC::km1
k2	CGGTGCCCTGAATGAACTGC	Check tolC::km1

ISSN: 1475-2859