Table 1 Bacterial strains and plasmids used in this study

E. coli strains

 DH10B-T1R

(F⁻ λ⁻) mcrA Δmrr-hsdRMS-mcrBC φ80lacZDM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK rpsL (StrR) nupG tonA

Novagen

 BW25141

(F- λ-) Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), Δ(phoB-phoR)580, galU95, ΔuidA3::pir, recA1, endA9(del-ins)::FRT, rph-1, Δ(rhaD-rhaB)568, hsdR51

[46]

 UT5600

K-12 (F⁻ λ⁻) Δ(ompT-fepC)266

[45]

 UT5600ΔtolC::Km1

UT5600 ΔtolC::Km1

This study

 EPEC

EPEC O127:H6 strain E2348/69

[48]

 MG1655

K-12 (F⁻ λ⁻)

[20]

 EcM1

MG1655 ΔfimA-H

[21, 22]

 EcM1∆fliCD

MG1655ΔfimA-H ΔfliCD

[49]

 SIEC

EcM1-∆yeeJ::Ptac-eLEE2 ∆yra::Ptac-eLEE3 ∆yfc::Ptac-eEscD ∆yebT::Ptac-eLEE4 ∆yfaL::Ptac-eLEE1

[19]

 SIECΔp1

SIEC ∆yfaL:: ΔPtac-eLEE1*

[19]

 EcM1-eEtgA

EcM1 Δsfm::Ptac-etgA

This study

 SIEC-eEtgA

SIEC Δsfm::Ptac-etgA

This study

 SIECΔp1-eEtgA

SIECΔp1 Δsfm::Ptac-etgA

This study

 SIEC-eLEE5

SIEC Δflu::Ptac-eLEE5

[19]

 SIEC-eLEE5-eEtgA

SIEC-eLEE5 Δsfm::Ptac-etgA

This study

 SIEC-eLEE5ΔescN

SIEC-eLEE5 ΔescN

This study

Plasmids

 pACBSR

Cm^R; p15A ori, P_BAD I-SceI λRed genes

[50]

 pKD4

Amp^R, oriRγ, Km^R-FRT cassette

[46]

 pKD46

Amp^R, oriR101, P_BAD λRed genes

[46]

 pGEsfmPtacGFP

Km^R; R6K ori, sfm HRs, Ptac-gfp^TCD

[19]

 pGEsfmPtacEtgA

Km^R; R6K ori, sfm HRs, Ptac-etgA

This study

 pGEΔescN

Km^R; R6K ori, escN HRs

[42]

 pT3s-Bla

Tc^R, pBR-ori, Ptac-EspF20Bla

[39]

 pBAD18

KmR, pBR-ori, PBAD

[43]

 pBAD18EspF20Bla

KmR, pBAD18, PBAD-EspF20Bla

This study

 pBAD18Bla

KmR, pBAD18, PBAD-Bla

This study