Fig. 3

Motility and viability of EcM1-eEtgA and SIEC-eEtgA. A Quantification of the motility of different E. coli strains in LB-soft agar plates containing the inductor IPTG (0.1 mM). The histogram represents the relative percentages of motility using the motility of the control strain EcM1 as 100%. The non-motile strain EcM1ΔfliCD was used as a negative control. The means and standard errors from four independent experiments are shown. Statistical analysis was performed using the two-tailed Student’s t test. Asterisks (**) indicates p-value < 0.01. B Image of a representative motility assay on LB-soft agar with IPTG. C Growth curve of the indicated E. coli strains (EcM1, EcM1-eEtgA, SIEC, and SIEC-eEtgA) on LB supplemented with IPTG. D Viability test (serial tenfold dilutions to determine colony forming units of the cultures) of the indicated strains (left) on LB agar plates supplemented with IPTG or additionally containing 0.2% (w/v) SDS or 2.5 mg/ml deoxycholate (DOC), as indicated. An E. coli K-12 ΔtolC::Km1 strain susceptible to SDS and DOC was used as a control