Fig. 2

Secretion of translocator proteins EspA, EspB, EspD and hemolytic activity by SIEC-eEtgA. A Coomassie-stained SDS-PAGE of proteins found in culture supernatans of the indicated strains (EcM1, EcM1-eEtgA, SIEC, and SIEC-eEtgA). Bands corresponding to the proteins FliC, EspD, EspB and EspA are labeled. Mass (in kDa) of protein standards is indicated to the left. B Relative quantification of secreted EspA and EspB translocator proteins in SIEC-eEtgA relative to their levels in SIEC (considered 100%). Histogram shows the means and standard errors of the relative percentage of the secretion of each protein. Data were obtained from nine independent experiments. C Histogram showing the relative hemolytic activity of the indicated strains (SIEC, SIECΔp1, SIEC-eEtgA, and SIECΔp1-eEtgA). The hemolysis obtained with a control strain (EcM1) was subtracted from the represented values. SIEC hemolysis values were taken as 100%. Data represent the means and standard errors of three independent experiments. Statistical analysis was performed using the two-tailed Student’s t test. Asterisks indicate p-value < 0.01 (**) and < 0.001 (***)