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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Enhanced protein translocation to mammalian cells by expression of EtgA transglycosylase in a synthetic injector E. coli strain

Fig. 1

The T3SS injectisome of EPEC and the encoding operons in the engineered SIEC strains. A Scheme of the T3SS structure of EPEC. The lytic transglycosylase EtgA, which is associated with peptidoglycan, is marked with a red ellipse. B Synthetic transcriptional units of the T3SS under the control of the tac promoter (Ptac) in the strains SIEC, SIEC-eEtgA and EcM1-eEtgA. The chromosomal loci used as integration sites of the eLEE operons are indicated to the right of each transcriptional unit (described in [19] and in this study for eEtgA in the sfm locus). Different colors of injectisome components in A correspond to loci in B

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