Enhanced production of terrein in marine-derived Aspergillus terreus by refactoring both global and pathway-specific transcription factors

Table 3 Primers used in this study

Name	Sequence	Use
PyrGF	GCCTCAAACAATGCTCTTCACCC	Amplification of pyrG
PyrGR	CAGAAAGAGTCACCGGTCACTGT ACGTCTGAGAGGAGGCACTGATGC	Amplification of pyrG
GPDF	GTACAGTGACCGGTGACTCTTTCTG	Amplification of promoter
GPDR	GGTGATGTCTGCTCAAGCGGGGTAG	Amplification of promoter
terR2	TCCCTTCATACCTCGCTTTA	Amplification of terR
GPDterRF	CCGCTTGAGCAGACATCACCATGTTCGCCGAACTTAACGC	Amplification of terR
terR1	TTTGAGGCTTATACAACGAG	Amplification of terR
terR2	AGACGCTTGTAGCCCGTTAT	Amplification of terR
TerARTF	AGCAGCAAGTCATACAGCAA	Expression analysis
TerARTR	AACTCCTCGCATAACAAAGC	Expression analysis
TerBRTF	CAAGGATTGAAGGCGGGATC	Expression analysis
TerBRTR	AGAAGTTTCGGGCACAGGTT	Expression analysis
TerCRTF	GCATCTGCAAAGCCTCCTGT	Expression analysis
TerCRTR	CTCTGGCGTGTCTCATACCG	Expression analysis
TerDRTF	TCAGGCTGGAGACAGTAATC	Expression analysis
TerDRTR	AACGCATCTGGTATCTGGTC	Expression analysis
TerERTF	TGGTGCCTCCCGAGAAACAG	Expression analysis
TerERTR	CTCGCCGACCTTGGCTACAT	Expression analysis
TerFRTF	TACGACCAGGAAGGAACAGT	Expression analysis
TerFRTR	GAGCCGATAGTAGCAGAAGC	Expression analysis
TerRRTF	CCCGACTCAGCAAGACGAAG	Expression analysis
TerRRTR	TGAAAGCCGAAAGCCATCAC	Expression analysis

ISSN: 1475-2859