Fig. 5

Bioactivities determination of RGDS, IL-15, and ADH in the SHTXTHHly fusion form. U87 MG cells were incubated with SHTRGDSTHHly/SHTRGESTHHly, mouse anti-S tag antibody, and the second antibody in sequence, then the cell staining was measured by FACS (A). U87 MG cells were incubated with SHTRGDSTHHly/SHTRGESTHHly and transferred into a fibronectin-coated plate. At different times, the attached cells were subjected to the CCK-8 reagent for quantification (B). In other experiments, cells were stained with DiO dye (green) and observed with a fluorescence microscope (C). Mo7e cells were incubated with different concentrations (1 pM-100 nM) of IL-15 and SHTIL15THHly for 4 days, and the cell numbers were measured with a CCK-8 reagent (D). The activities of ADH and SHTADHTHHly were compared using Ethyl Acetoacetate as the substrate and NADH as the co-factor (E). The activity was measured at 340 nm to monitor the decrease in the absorbance of NADH quantitatively. S: Slope. The 1-tailed Student's t-test was used to determine statistical differences between the two groups