Skip to main content
Fig. 4 | Microbial Cell Factories

Fig. 4

From: SHTXTHHly, an extracellular secretion platform for the preparation of bioactive peptides and proteins in Escherichia coli

Fig. 4

Bioactivities determination of the produced AMPs. Different concentrations (0–4000 nM) of cCeA and rCeA (A), or cLL37 and rLL37 (B), were incubated with E. coli 25,922 at 37 °C for 18 h, then the OD600 values were detected. Different concentrations (0–64 μM) of cA12 and rA12 (C) were incubated with U87 MG cells for 8 h, and the number of live cells was measured with the CCK-8 reagent. In other experiments, the concentration of A12 and the incubation time were set at 10 μM and 4 h, then the tumor lysis (D) and pro-apoptosis (E) effect were verified with a fluorescence microscope or an Annexin V-FITC/PI dye

Back to article page