Fig. 3

Production of the antimicrobial peptides using the SHTXTHHly system. A SDS-PAGE analysis of culture samples (n = 2). W: Whole-cell lysate; S: Supernatant. The native and S tag-fused tag fusion proteins are indicated above the image. The arrows indicate the tag fusion proteins. B Schematic diagram of the purification procedure. After TEV enzyme digestion, the un-cut fusion proteins, the digested fragments, and the His tag conjugated TEV enzyme would be caught by the nickel column, and only the target proteins would be flowed through. C SDS-PAGE analysis of the purification of PEW300. S: Supernatant; FT: Flow-through; E: Eluent; U-E: Ultra-filtration product of the eluent; D-U: TEV enzyme digestion production of the ultra-filtration product; 1: First round nickel affinity chromatography; 2: Second round nickel affinity chromatography. All SDS-PAGE was conducted under non-reducing conditions