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Fig. 1 | Microbial Cell Factories

Fig. 1

From: SHTXTHHly, an extracellular secretion platform for the preparation of bioactive peptides and proteins in Escherichia coli

Fig. 1

Positive correlation between the pI value and the secretion level. A Schematic diagram of the XTHHly system. POI: Protein of interest; TEV cs: TEV enzyme cleavage site; Hly218: the last 218 amino acids of hemolysin A. BD SDS-PAGE analysis of culture samples (n = 2). W: Whole-cell lysate; S: Supernatant. The native and mutated tag fusion proteins are indicated above the image. The arrows indicate the tag fusion proteins. C–E The relationship between the pI value and the secretion level. The secretion levels of mutated tag fusion proteins were estimated by Image J scanning. All SDS-PAGE was conducted under non-reducing conditions

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