Table 1 Strains and plasmids used in this study
Strains or plasmids | Description | Reference |
|---|---|---|
Strains | ||
E. coli JM109 | Clone strain | Takara |
Pontibacillus sp. ZY | Clone strain | Our laboratory |
B. subtilis 168 | trpC2, clone strain | Our laboratory |
B. subtilis WB600 | B. subtilis 168 derivate, deficient in nprE, aprE, epr, bpr, mpr, nprB, expression strain | Our laboratory |
BZ1 | B. subtilis WB600/pBHE | This work |
BZ2 | B. subtilis WB600/pBHP | This work |
BZ3 | B. subtilis WB600/pBHS | This work |
BZ4 | B. subtilis WB600/pBHA | This work |
BZ5 | B. subtilis WB600/pBHO | This work |
BZ6 | B. subtilis WB600/pBHL | This work |
BZd1 | B. subtilis WB600/pBHES | This work |
BZd2 | B. subtilis WB600/pBHPS | This work |
BZd3 | B. subtilis WB600/pBHSS | This work |
BZd31 | B. subtilis WB600/pBHSS1 | This work |
BZd32 | B. subtilis WB600/pBHSS2 | This work |
BZd33 | B. subtilis WB600/pBHSS3 | This work |
BZd34 | B. subtilis WB600/pBHSS4 | This work |
BZd35 | B. subtilis WB600/pBHSS5 | This work |
BZd36 | B. subtilis WB600/pBHSS6 | This work |
BZd37 | B. subtilis WB600/pBHSS7 | This work |
BZd341 | B. subtilis WB600/pBHSS8 | This work |
BZd342 | B. subtilis WB600/pBHSS9 | This work |
BZd343 | B. subtilis WB600/pBHSS10 | This work |
BZd344 | B. subtilis WB600/pBHSS11 | This work |
BZd345 | B. subtilis WB600/pBHSS12 | This work |
Plasmids | ||
pHT43 | Ampr (E. coli), Cmr (B. subtilis), PgroE, SPamyQ | [44] |
pBEP43 | Ampr (E. coli), Kanr (B. subtilis), P43, SPamyQ | [45] |
pBHE | pHT43 derivative, PgroE, SPamyQ | This work |
pBHP | pHT43 derivative, P43, SPamyQ | This work |
pBHS | pHT43 derivative, PspoVG, SPamyQ | This work |
pBHA | pHT43 derivative, PsecA, SPamyQ | This work |
pBHO | pHT43 derivative, PodhA, SPamyQ | This work |
pBHL | pHT43 derivative, PlytR, SPamyQ | This work |
pBHES | pHT43 derivative, PgroE-PspoVG, SPamyQ | This work |
pBHPS | pHT43 derivative, P43-PspoVG, SPamyQ | This work |
pBHSS | pHT43 derivative, PspoVG-PspoVG, SPamyQ | This work |
pBHSS1 | pHT43 derivative, PspoVG-PspoVG1, SPamyQ | This work |
pBHSS2 | pHT43 derivative, PspoVG-PspoVG2, SPamyQ | This work |
pBHSS3 | pHT43 derivative, PspoVG-PspoVG42, SPamyQ | This work |
pBHSS4 | pHT43 derivative, PspoVG-PspoVG142, SPamyQ | This work |
pBHSS5 | pHT43 derivative, PspoVG-PspoVG242, SPamyQ | This work |
pBHSS6 | pHT43 derivative, PspoVG42-PspoVG142, SPamyQ | This work |
pBHSS7 | pHT43 derivative, PspoVG42-PspoVG242, SPamyQ | This work |
pBHSS8 | pHT43 derivative, PspoVG-PspoVG142, SPopt1 | This work |
pBHSS9 | pHT43 derivative, PspoVG-PspoVG142, SPopt2 | This work |
pBHSS10 | pHT43 derivative, PspoVG-PspoVG142, SPopt3 | This work |
pBHSS11 | pHT43 derivative, PspoVG-PspoVG142, SPopt4 | This work |
pBHSS12 | pHT43 derivative, PspoVG-PspoVG142, SPopt5 | This work |