Fig. 3

DNA replication run-out histograms of Escherichia coli derivatives in LB. Chromosome contents of each E. coli strain at the stationary phase (A) and exponential phase in LB (B–E) were determined using flow cytometry analysis. Two major peaks of Hoechst fluorescence indicated that E. coli cells contained four or eight chromosomes (B–D). Four to five major peaks with indistinguishable minor peaks of Hoechst fluorescence indicated that E. coli cells contained several copies of the chromosomes (E). A Wild-type cells in the stationary phase. B Wild-type cells in the exponential phase. C O2 lacZ cells in the exponential phase. D O3 lacZ dadX cells in the exponential phase. E O2’ lacZ dadX cells in the exponential phase