Fig. 1
From: The TetR-type regulator AtsR is involved in multidrug response in Corynebacterium glutamicum
β-galactosidase activity driven by the promoter of the ncgl0887-atsR operon. A, B β-galactosidase activity analysis of the promoter was performed using the transcriptional Pncgl0887::lacZY chromosomal fusion reporter expressed in WT(pXMJ19), ΔatsR(pXMJ19), ΔatsR(pXMJ19-atsR), and ΔatsR(pXMJ19-atsR:C123SC187S)(the ΔatsR mutant expressed the pXMJ19-atsR:C123SC187S) strains exposed to streptomycin (STR) or hydrogen peroxide (H2O2) for 30 min. β-galactosidase activity analysis of the promoter in WT(pXMJ19-atsR) strains was performed in the absence of agents. C, D Fold change of the transcription level was calculated according to the data from Fig. 1A, B by the equation: the value obtained from the strains exposed to stress/the value obtained from the corresponding strains without stress