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Fig. 7 | Microbial Cell Factories

Fig. 7

From: Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression

Fig. 7

Testing expression of P. putida KT2440 PIbpA-PET hydrolase knock-in strains. A Experimental protocol to test the expression of genomically integrated PIbpA-PET hydrolase constructs upon different heat shock regimes. B OD600 and esterase activities of P. putida KT2440 (wt) or the corresponding PIbpA-EhaA-LCC, PIbpA-bglx-HiC, and PIbpA-lasb-IsPDura knock-in strains in dependance on the heat shock regime. Data depict one representative experiment with duplicate cultures

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