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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression

Fig. 6

Defining PIbpA temperature-dependent expression output. A Experimental protocol to test PET hydrolase expression from PIbpA upon different heat shock regimes. B OD600 and esterase activities of P. putida TA7-EG cultures transformed with pLo-PIbpA (empty vector, e.v.). or pLo-PIbpA-bglx-HiC in dependance on the heat shock regime. Data shown are derived from one representative experiment with duplicate cultures

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