Fig. 5From: Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expressionTesting PL and PIbpA heat shock inducible promoters. A Overview of the cI857/PL system; this transcriptional regulatory unit spans 1410Â bp from the PacI to the AvrII site. B Sequence overview and relevant features of the PIbpA system used here. C Experimental protocol to test PET hydrolase expression from PL and PIbpA upon heat shock. D OD600 of P. putida TA7-EGÂ cultures expressing PET hydrolases in dependance on the heat shock regime. E Esterase activity of cultures expressing PET hydrolases in dependance on the heat shock regime; top, esterase activity in the supernatant; bottom, cell-associated esterase activity. Data shown are derived from one representative experiment with duplicate culturesBack to article page