Fig. 5

Testing P_L and P_IbpA heat shock inducible promoters. A Overview of the cI857/P_L system; this transcriptional regulatory unit spans 1410 bp from the PacI to the AvrII site. B Sequence overview and relevant features of the P_IbpA system used here. C Experimental protocol to test PET hydrolase expression from P_L and P_IbpA upon heat shock. D OD600 of P. putida TA7-EG cultures expressing PET hydrolases in dependance on the heat shock regime. E Esterase activity of cultures expressing PET hydrolases in dependance on the heat shock regime; top, esterase activity in the supernatant; bottom, cell-associated esterase activity. Data shown are derived from one representative experiment with duplicate cultures