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Fig. 3 | Microbial Cell Factories

Fig. 3

From: Towards synthetic PETtrophy: Engineering Pseudomonas putida for concurrent polyethylene terephthalate (PET) monomer metabolism and PET hydrolase expression

Fig. 3

Testing PET hydrolase membrane display systems. A Overview of combinatorial test construct design for PET hydrolase membrane expression, including plasmids providing low, medium, and high cellular copy number, transcriptional control by the PrhaB system, translation initiation via the BCD2 translational coupler, three different membrane display anchors, and three different PET hydrolases. B OD600 and esterase activity of P. putida TA7-EG shake flask cultures expressing the indicated display constructs from the RK2 low copy number plasmid, measured for 5 h post-induction (induction at t = 0 h). C OD600 and esterase activity of P. putida TA7-EG shake flask cultures expressing the indicated display constructs from the pBBR1 medium copy number plasmid, measured for 5 h post-induction (induction at t = 0 h). The * in (B, C) indicates that esterase activity was not measured at the t = 5 h timepoint due to obvious cell lysis. Data shown are derived from one representative experiment with duplicate cultures

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