Fig. 2

Engineering TA, EG and BD metabolism in P. putida. A Overview of the engineering steps, including genomic insertion (yellow) and ALE (red), to endow P. putida with the capacity to use TA, EG and BD as sole carbon sources. B Growth of P. putida KT2440 and P. putida TA in shake flasks with M9 minimal medium, 0.4% TA, pH 5.5. C Growth of P. putida TA and P. putida TA7 in shake flasks with M9 minimal medium, 0.4% TA, pH 5.5 or pH 7.5. D Growth of P. putida TA7 and P. putida TA7-EG analyzed in a plate reader assay with M9 minimal medium, 10 mM EG or 10 mM EG + 10 mM TA, pH 7.5. E Growth of P. putida TA7 and P. putida TA7-BD analyzed in a plate reader assay with M9 minimal medium, 10 mM BD or 10 mM BD + 10 mM TA, pH 7.5. Data shown are mean and SD, n = 2 (B and C) or n = 6 (D and E)