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Table 2 Oligonucleotides used in this study

From: PhoP- and GlnR-mediated regulation of metK transcription and its impact upon S-adenosyl-methionine biosynthesis in Saccharopolyspora erythraea

Oligonucleotide

Sequence (5′–3′)a

Primers for construction ΔglnR strain

 F-glnR-up

CCCAAGCTTCACCGGCGATGTTGACCGACCCGTC

 R-glnR-up

GCTCTAGAGAGGAGGGCCTCCATCCCAGGGCGG

 F-glnR-dw

CGGGGTACCGTTCGAAGCGTGCAGCTCACCTGG

 R-glnR-dw

CCGGAATTCGCGAGTCCGAGCCGCCGAAGTCGAT

Primers for construction OglnR strain

 F-OglnR

GGAATTCCATATGATGAGCTCTGAGCTTCTCCTGCTC

 R-OglnR

TGCAGGATATCTCAGCGGACGACCGCGG

Primers for construction OphoP strain

 F-OphoP

GGAATTCCATATGGTGACCAGGGTGCTGATCGTG

 R-OphoP

GACGATATCCTACACCTCGAACTTGTAGCCGA

Primers for biotin labeling EMSAs probe

 Bio-primer

Biotin-AGCCAGTGGCGATAAG

Primers for EMSAs

 SACE_2103F

AGCCAGTGGCGATAAGCCGCAAGGCCTGCGAC

 SACE_2103R

AGCCAGTGGCGATAAGGCTGATCGCGTCGCAGATC

 SACE_3900F

AGCCAGTGGCGATAAGAGACCGTGGACTTCGAGGATCTCAG

 SACE_3900R

AGCCAGTGGCGATAAGATGGAGTCGCTGATCGCGTCG

 SACE_3900-1F

AGCCAGTGGCGATAAGAGACCGTGGACTTCGAGGATCTCAGCG

 SACE_3900-1R

AGCCAGTGGCGATAAGATGCGGTCGGAGAGCTCCTGTTCG

 SACE_3900-2F

AGCCAGTGGCGATAAGCGGCGAGGATTACCGGAACCGG

 SACE_3900-2R

AGCCAGTGGCGATAAGAGACTGCGCATGGAAGATCCCCCAC

 SACE_3900-3F

AGCCAGTGGCGATAAGTGACAGTGACCGTCCGTACGCAAGGATCG

 SACE_3900-3R

AGCCAGTGGCGATAAGCGACTCACTCGTGAACAACCTGCGGTTGATC

Primers for PCR

 RT6965-F

AGCGGGACCGACGTCTGC

 RT6965-R

TGACCTCCTCGCCGGAGAC

 RT7101-F

CGCCGAGTGGGGTGTGG

 RT7101-R

CCGAAGAAGTCGTAGCCCCAG

 RT3900-F

GTCATCGACGAGATCGCCAAGCAG

 RT3900-R

GTGCTGGGTGGACAGCACCACC