Oligonucleotide | Sequence (5′–3′)a |
---|---|
Primers for construction ΔglnR strain | |
 F-glnR-up | CCCAAGCTTCACCGGCGATGTTGACCGACCCGTC |
 R-glnR-up | GCTCTAGAGAGGAGGGCCTCCATCCCAGGGCGG |
 F-glnR-dw | CGGGGTACCGTTCGAAGCGTGCAGCTCACCTGG |
 R-glnR-dw | CCGGAATTCGCGAGTCCGAGCCGCCGAAGTCGAT |
Primers for construction OglnR strain | |
 F-OglnR | GGAATTCCATATGATGAGCTCTGAGCTTCTCCTGCTC |
 R-OglnR | TGCAGGATATCTCAGCGGACGACCGCGG |
Primers for construction OphoP strain | |
 F-OphoP | GGAATTCCATATGGTGACCAGGGTGCTGATCGTG |
 R-OphoP | GACGATATCCTACACCTCGAACTTGTAGCCGA |
Primers for biotin labeling EMSAs probe | |
 Bio-primer | Biotin-AGCCAGTGGCGATAAG |
Primers for EMSAs | |
 SACE_2103F | AGCCAGTGGCGATAAGCCGCAAGGCCTGCGAC |
 SACE_2103R | AGCCAGTGGCGATAAGGCTGATCGCGTCGCAGATC |
 SACE_3900F | AGCCAGTGGCGATAAGAGACCGTGGACTTCGAGGATCTCAG |
 SACE_3900R | AGCCAGTGGCGATAAGATGGAGTCGCTGATCGCGTCG |
 SACE_3900-1F | AGCCAGTGGCGATAAGAGACCGTGGACTTCGAGGATCTCAGCG |
 SACE_3900-1R | AGCCAGTGGCGATAAGATGCGGTCGGAGAGCTCCTGTTCG |
 SACE_3900-2F | AGCCAGTGGCGATAAGCGGCGAGGATTACCGGAACCGG |
 SACE_3900-2R | AGCCAGTGGCGATAAGAGACTGCGCATGGAAGATCCCCCAC |
 SACE_3900-3F | AGCCAGTGGCGATAAGTGACAGTGACCGTCCGTACGCAAGGATCG |
 SACE_3900-3R | AGCCAGTGGCGATAAGCGACTCACTCGTGAACAACCTGCGGTTGATC |
Primers for PCR | |
 RT6965-F | AGCGGGACCGACGTCTGC |
 RT6965-R | TGACCTCCTCGCCGGAGAC |
 RT7101-F | CGCCGAGTGGGGTGTGG |
 RT7101-R | CCGAAGAAGTCGTAGCCCCAG |
 RT3900-F | GTCATCGACGAGATCGCCAAGCAG |
 RT3900-R | GTGCTGGGTGGACAGCACCACC |