Table 1 Strains and plasmids used in this study
Strain or plasmid | Relevant characteristic (s) | Source or references |
|---|---|---|
Strains | ||
 S. erythraea NRRL2338 | Wild-type strain | DSM |
 ΔglnR | glnR knockout strain | [20] |
 OglnR | glnR overexpression strain | [30] |
 OphoP | phoP overexpression strain | [2] |
 ΔglnR::glnR | glnR complementary strain | [30] |
E. coli | ||
 DH5α | F¯Ф80dlacZΔM(lacZYA-argF)U169 deoR | GIBCO-BRL |
 BL21(DE3) | F′ompT hsdS gal dcm (DE3) | Novagen |
Plasmids | ||
 pET-28a | Expression vector with T7 promoter | Novagen |
 pET-glnR | pET28a with glnR | [20] |
 pET-phoP | pET28a with phop | [2] |
 pIB139 | E. coli-S. erythraea integrative shuttle vector containing ermE promoter | Lab stock |
 pIB-glnR | pIB139 with glnR for gene overexpression | Lab stock |
 pIB-phoP | pIB139 with phoP for gene overexpression | Lab stock |