Fig. 4

PhoP and GlnR promote SAM production in S. erythraea. A Content of SAM in WT and OphoP strains. B Content of SAM in WT, ΔglnR, ΔglnR::glnR and OglnR strains. The WT and OphoP were grown in phosphate-limiting Evans, while WT, ΔglnR, ΔglnR::glnR and OglnR were grown in nitrogen-limiting Evans. Error bars indicate standard deviations from three independent biological replicates. *P < 0.05 by One-way ANOVA and Tukey’s multiple comparisons test using GraphPad Prism 8