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Fig. 3 | Microbial Cell Factories

Fig. 3

From: PhoP- and GlnR-mediated regulation of metK transcription and its impact upon S-adenosyl-methionine biosynthesis in Saccharopolyspora erythraea

Fig. 3

PhoP and GlnR positively regulate the expression of SACE_3900. A RT-qPCR analysis of phoP and SACE_3900 in WT and OphoP following 36 h or 72 h of growth. B Gene transcription of glnR and SACE_3900 in WT, ΔglnR, ΔglnR::glnR, OglnR strain at 36 and 72 h. The WT and OphoP were grown in phosphate-limiting Evans (40 μM KH2PO4 and C and N as basic), while WT, ΔglnR, ΔglnR::glnR and OglnR were grown in nitrogen-limiting Evans [1 mM (NH4)2SO4 and C and P as basic]. Error bars indicate standard deviations from three independent biological replicates

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