Fig. 1

Interaction between PhoP or GlnR and the promoter region of putative metK genes. A Schematic representation of the genetic positioning of putative metK genes in S. erythraea, and the possible regulatory effect of PhoP and GlnR. B Interaction assessment for His₆-PhoP and the upstream promoter region of SACE_2103 (left) and SACE_3900 (right). C Interaction assessment for His₆-GlnR and the upstream promoter region of SACE_2103 (left) and SACE_3900 (right). Concentration of His₆-tagged PhoP and GlnR protein was (Lane 1, 0; Lane 2, 0.2; and Lane 3, 0.4 μM). Arrow heads show the free probes without bound protein. Unlabeled specific probe, S; non-specific competitor DNA, N; DNA/pantothenate metabolism flavoprotein, dfp; hydrolase (secreted trypsin-like serine protease), hyd; S-adenosyl-L-homocysteine hydrolase, sahH; 5-methyltetrahydrofolate–homocysteine methyltransferase, metH; adenosine kinase, adoK; S-adenosylmethionine synthetase, metK; glycine sarcosine N-methyltransferase, met1; acetyl-CoA synthetase (AMP-dependent synthetase/ligase), acs.