Table 2 RBS sequences for RBS libraries with defined TIR
RBS | ORF | T.I.R | Sequences (5’-3’) |
|---|---|---|---|
MVK1 | MVK | 1017.7 | TGCAAATTTATATCTACGTTAAAAATACTATCAAT |
MVK2 | MVK | 2017 | TGCAAATTTATATCTACGTTCGGAGCAACTTCAAT |
MVK3 | MVK | 2952.5 | TGCAAATTTATATCTACGTTCGTAGCGAGTTCAAT |
MVK5 | MVK | 5078.99 | TGCAAATTTATATCTACGTCAGAGTCGGGTTCAAT |
MVK11 | MVK | 11418.02 | TGCAAATTTATATCTACGTTATAAGGAAAGTCAAT |
MVK15 | MVK | 15646.14 | TGCAAATTTATATCTACGTTATAAGGAGCTTCAAT |
MVK20 | MVK | 20447.37 | TGCAAATTTATATCTACGTTATAAAGCAGGTCAAT |
MVK30 | MVK | 30731.61 | TGCAAATTTATATCTACGTTTAAAGGAACCTCAAT |
MVK43 | MVK | 46662.15 | TGCAAATTTATATCTACGTAAAGGAGCTTCTCAAT |
MVK60 | MVK | 62989.43 | TGCAAATTTATATCTACGTTTTAGGGAGAGTCAAT |
PMK1 | PMK | 1002.56 | AGGCCTCATAATAAATCAACATCACGGAGCGAGAA |
PMK2 | PMK | 2041.67 | AGGCCTCATAATAAATCAAGAAGGTCGGAGGAGAA |
PMK3 | PMK | 3095.85 | AGGCCTCATAATAAATCAATCAGGGGAGTAGAGAA |
PMK5 | PMK | 4822.1 | AGGCCTCATAATAAATCAAGTCCAGGGAGGGAGAA |
PMK10 | PMK | 9851.05 | AGGCCTCATAATAAATCAAGGGAGGTTACAGAGAA |
PMK15 | PMK | 14957.6 | AGGCCTCATAATAAATCAAGCTAAGGAGGAGAGAA |
PMK20 | PMK | 20355.56 | AGGCCTCATAATAAATCAAAGAACAGGAGGGAGAA |
PMK30 | PMK | 30658.04 | AGGCCTCATAATAAATCAAGTTAGAGGAGGGAGAA |
PMK42 | PMK | 42010.79 | AGGCCTCATAATAAATCAATAGTAAGGAGGGAGAA |
PMK53 | PMK | 53024.08 | AGGCCTCATAATAAATCAATAGAGGAGGTCGAGAA |
MVD1 | MVD | 992.38 | AGATTGAAAATTCCCAGACAGTCTCAGCCCGCACA |
MVD2 | MVD | 1995.95 | AGATTGAAAATTCCCAGACAAAATCGGATAGCACA |
MVD3 | MVD | 3112.61 | AGATTGAAAATTCCCAGACATAATTTCTAAGCACA |
MVD5.5 | MVD | 5495.17 | AGATTGAAAATTCCCAGACCCACTAAGCTCGCACA |
MVD10 | MVD | 10029.99 | AGATTGAAAATTCCCAGACCTAAGATTACAGCACA |
MVD15 | MVD | 15844.55 | AGATTGAAAATTCCCAGACTTAGGACCTTCGCACA |
MVD20 | MVD | 20887.54 | AGATTGAAAATTCCCAGACTCACAGAGGACGCACA |
MVD30 | MVD | 29379.21 | AGATTGAAAATTCCCAGACAGAAGGCAGCCGCACA |
MVD43 | MVD | 42645.61 | AGATTGAAAATTCCCAGACCGAGGATACCAGCACA |
MVD50 | MVD | 50417.64 | AGATTGAAAATTCCCAGACCCTAAGGCCCCGCACA |
IDI1 | IDI | 993.72 | AGAGTAAAAATAAGCCAAAACCCAAAGCAGCCTCA |
IDI2 | IDI | 1969.19 | AGAGTAAAAATAAGCCAAACGACGCTCAGACCTCA |
IDI3 | IDI | 3238.36 | AGAGTAAAAATAAGCCAAATCCTCACAGATCCTCA |
IDI5 | IDI | 5106.49 | AGAGTAAAAATAAGCCAAAGACACGAAGTTCCTCA |
IDI10 | IDI | 10029.99 | AGAGTAAAAATAAGCCAAACTAAGAAAACTCCTCA |
IDI16 | IDI | 16327.19 | AGAGTAAAAATAAGCCAAAACAGTAGGAACCCTC |
IDI20 | IDI | 20496.44 | AGAGTAAAAATAAGCCAAAATTAAAGAGATCCTCA |
IDI30 | IDI | 29751.76 | AGAGTAAAAATAAGCCAAATAAGGACTCTCCCTCA |
IDI48 | IDI | 48198.92 | AGAGTAAAAATAAGCCAAAGATAACGAGGTCCTCA |
IDI60 | IDI | 60579.68 | AGAGTAAAAATAAGCCAAAAAGGAGGAGAACCTCA |