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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Optimizing the downstream MVA pathway using a combination optimization strategy to increase lycopene yield in Escherichia coli

Fig. 1

Library construction and visual selection based on-plate and 96 deep-well plate selections. A Lycopene accumulation pathway in E. coli by heterogenous MVA pathway. The whole pathway was divided to three modules: module ESE, the upstream MVA pathway; module MPMI, the downstream MVA pathway; module EBI, the lycopene synthesis pathway containing Erg20, CrtE, CrtB, and CrtI, In the module2, an RBS library and combinatorial constructs based on three plasmid backbones (with the pT7 promoter and the p15A origin, with pTrc promoter and p15A origin, or with pTrc promoter and pSC101) were employed to screen the optimal expression levels for the four genes MVK, PMK, MVD, and IDI to achieve the purpose of optimizing the lycopene metabolic flux. B On-plate and 96-deep-well plate visual selection of pHM-library. Each colony on the plate or 96-deep-well plate showed different colors indicating different lycopene accumulation

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